The Escherichia coli NarL receiver domain regulates transcription through promoter specific functions
نویسندگان
چکیده
منابع مشابه
UTP allosterically regulates transcription by Escherichia coli RNA polymerase from the bacteriophage T7 A1 promoter.
In the case of Escherichia coli RNA polymerase, UTP at elevated concentrations suppresses terminated transcript accumulation during multiple-round transcription from a DNA construct containing the T7 A1 promoter and T(e) terminator. The step that is affected by UTP at elevated concentrations is promoter clearance. In an attempt to understand better the mechanism by which UTP regulates this step...
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The Escherichia coli lac and ara promoters and rrnC ribosomal RNA promoter-leader region were fused to lacZYA. Transcription termination signals were introduced into the lac genes of these fusions by Tn9 and IS1 insertions. Measurement of lac enzymes from upstream and downstream of the insertions showed that termination signals resulting from these insertions are very efficient when transcripti...
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We present here a computational analysis showing that sigma70 house-keeping promoters are located within zones with high densities of promoter-like signals in Escherichia coli, and we introduce strategies that allow for the correct computer prediction of sigma70 promoters. Based on 599 experimentally verified promoters of E.coli K-12, we generated and evaluated more than 200 weight matrices opt...
متن کاملThe Escherichia coli K-12 NarL and NarP proteins insulate the nrf promoter from the effects of integration host factor.
The Escherichia coli K-12 nrf operon promoter can be activated fully by the FNR protein (regulator of fumarate and nitrate reduction) binding to a site centered at position -41.5. FNR-dependent transcription is suppressed by integration host factor (IHF) binding at position -54, and this suppression is counteracted by binding of the NarL or NarP response regulator at position -74.5. The E. coli...
متن کاملLocation of sequences in the nar promoter of Escherichia coli required for regulation by Fnr and NarL.
Nitrate reductase, encoded by the nar operon in Escherichia coli, is produced only under anaerobic conditions and induced to its maximum level in the presence of nitrate. The anaerobic expression of the nar operon depends on the fnr gene product (Fnr), and the stimulation of anaerobic expression by nitrate requires the narL gene product (NarL). Distinct regulatory domains within the nar promote...
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2015
ISSN: 1471-2180
DOI: 10.1186/s12866-015-0502-9